Construction of a GLUT-1 and HIF-1α gene knockout cell model in HEp-2 cells using the CRISPR/Cas9 technique
Zhong-Jie Lu,1 Qi Yu,2 Shui-Hong Zhou,2 Jun Fan,3 Li-Fang Shen,2 Yang-Yang Bao,2 Ting-Ting Wu,2 Min-Li Zhou,2 Ya-Ping Huang3 1Department of Radiotherapy, 2Department of Otolaryngology, 3State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang